KMID : 1084220170240050271
|
|
Journal of Rheumatic Diseases 2017 Volume.24 No. 5 p.271 ~ p.278
|
|
Interleukin-17 Enhances Germinal Center Formation and Immunoglobulin G1 Production in Mice
|
|
Lee Jennifer
Lee Seon-Young Kang Chang-Min Jhun Joo-Yeon Kim Ji-Hun Cho Mi-La Park Sung-Hwan Kim Ho-Youn Kwok Seung-Ki
|
|
Abstract
|
|
|
Objective. Interleukin (IL)-17 is a pro-inflammatory cytokine that has pleiotropic effects on multiple target cells and thereby contributes to the development of immune-mediated inflammatory disorders. However, the role of IL-17 in the humoral immune response has not been clearly elucidated.
Methods. Mice deficient in IL-17A (IL-17A knockout [KO] mice) and wild type (WT) C57BL/6 mice were compared. Distinct B cell (mature/precursor and marginal zone/follicular) and plasma cell populations were compared using fluorescence-activated cell sorting (FACS) and confocal immunostaining. Immunoglobulin production was assessed by enzyme-linked immunosorbent assay.
Results. There was no difference in B cell and plasma cell populations between IL-17A KO and WT mice. However, after T cell-dependent antigen challenge, IL-17A KO mice produced lower levels of immunoglobulin (Ig)G1 than wild-type animals. IL-17A KO mice also showed reduced germinal center (GC) formation and lower expression of activation-induced cytidine deaminase, the essential enzyme for class switch recombination (CSR). IL-17 had no effect on the proliferation or survival of naive B cells in in vitro functional studies. However, IL-17 treatment promoted naive B cell differentiation into plasma cells in synergy with IL-4, although IL-17 alone had no effect.
Conclusion. Our findings suggest that IL-17 contributes to the humoral immune response by enhancing GC formation, CSR to IgG1, and plasma cell differentiation in synergy with IL-4.
|
|
KEYWORD
|
|
Interleukin-17, B cell, Germinal center
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|